Definition of sonicate in English:

sonicate

verb ˈsɒnɪkeɪtˈsänikāt

[with object]Biochemistry

Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes.
用超声波使（生物样品）降解，超声处理
cells were sonicated in an ice bath
Example sentencesExamples
- Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity.
- The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.
- The sample was sonicated to clarity in a sonication bath (Ultrawave).
- After dialysis the residue was sonicated and ready for use.
- The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.

noun ˈsɒnɪkeɪtˈsänikāt

Biochemistry

A biological sample which has been sonicated.
超声波降解标本
Example sentencesExamples
- Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions.
- Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
- The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
- The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
- The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).

Derivatives

sonication

noun

Biochemistry

Cells were disrupted by sonication, and soluble recombinant proteins were purified on the TALON metal-affinity resin (Clontech).
Example sentencesExamples
- Lipid hydration was coupled with six cycles of freezing, thawing, and sonication, except sonication was omitted in the last cycle.
- Chromatin was sheared by sonication, and DNA associated with either CLF1 or ORC1 was separately co-immunoprecipitated using an anti-HA antibody.
- To test whether the brief low-power sonication changes the solubility limit of cholesterol in a bilayer, we performed a control experiment with 16: 0,18: 1PC.
- The following day, cells were harvested by centrifugation, resuspended in buffer (20 mM Tris-HCl, pH 8.0; 100 mM NaCl), and disrupted by sonication.

Origin

1950s: from sonic + -ate².

Definition of sonicate in US English:

sonicate

verbˈsänikāt

[with object]Biochemistry

Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes.
用超声波使（生物样品）降解，超声处理
cells were sonicated in an ice bath
Example sentencesExamples
- The sample was sonicated to clarity in a sonication bath (Ultrawave).
- The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.
- After dialysis the residue was sonicated and ready for use.
- The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.
- Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity.

nounˈsänikāt

Biochemistry

A biological sample which has been sonicated.
超声波降解标本
Example sentencesExamples
- The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
- The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).
- The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
- Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
- Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions.

Origin

1950s: from sonic + -ate.

单词	sonicate
释义	Definition of sonicate in English: sonicate verb ˈsɒnɪkeɪtˈsänikāt [with object]Biochemistry Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes. 用超声波使（生物样品）降解，超声处理 cells were sonicated in an ice bath Example sentencesExamples Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity. The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter. The sample was sonicated to clarity in a sonication bath (Ultrawave). After dialysis the residue was sonicated and ready for use. The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above. noun ˈsɒnɪkeɪtˈsänikāt Biochemistry A biological sample which has been sonicated. 超声波降解标本 Example sentencesExamples Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions. Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded. The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C. The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected. The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer). Derivatives sonication noun Biochemistry Cells were disrupted by sonication, and soluble recombinant proteins were purified on the TALON metal-affinity resin (Clontech). Example sentencesExamples Lipid hydration was coupled with six cycles of freezing, thawing, and sonication, except sonication was omitted in the last cycle. Chromatin was sheared by sonication, and DNA associated with either CLF1 or ORC1 was separately co-immunoprecipitated using an anti-HA antibody. To test whether the brief low-power sonication changes the solubility limit of cholesterol in a bilayer, we performed a control experiment with 16: 0,18: 1PC. The following day, cells were harvested by centrifugation, resuspended in buffer (20 mM Tris-HCl, pH 8.0; 100 mM NaCl), and disrupted by sonication. Origin 1950s: from sonic + -ate². Definition of sonicate in US English: sonicate verbˈsänikāt [with object]Biochemistry Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes. 用超声波使（生物样品）降解，超声处理 cells were sonicated in an ice bath Example sentencesExamples The sample was sonicated to clarity in a sonication bath (Ultrawave). The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above. After dialysis the residue was sonicated and ready for use. The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter. Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity. nounˈsänikāt Biochemistry A biological sample which has been sonicated. 超声波降解标本 Example sentencesExamples The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C. The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer). The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected. Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded. Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions. Origin 1950s: from sonic + -ate.
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