Definition of transfect in English:

transfect

verbtranzˈfɛkt-ns-trɑːnz-transˈfekt

[with object]Microbiology

1Infect (a cell) with free nucleic acid.
〔微生物〕使（细胞）感染病毒核酸，使转染
cells were transfected by electroporation
Example sentencesExamples
- Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d.
- Stably transfected cells were selected with 500 g/ml G418 (Sigma).
- Cells were transfected with eYFP-C10HRas 3-4 days before the experiment.
- Cells were grown to a density of 5 x 10 cells/ml and transfected by electroporation.
- MC57 cells were also transfected by electroporation and selected in 1.2 mg/ml G418.
1. 1.1 Introduce (genetic material) by infecting a cell with free nucleic acid.
  Example sentencesExamples
  It was known that there were certain ‘poison sequences’ which might exist upstream of the encoding area of the gene, which would be desirable to remove before transfecting the gene into COS cells.
  PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter.
  HEK293 cells were cultured in 100 × 20 mm regular culture dishes and transfected with 5 g DNA.
  I have purified, cloned, sub-cloned, transfected, blotted and detected DNA.
  An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid.

Derivatives

transfectant

noun

Microbiology

At 1 ng/ml MMC, no statistically significant difference was observed between wild-type, mutant and vector transfectants.
Example sentencesExamples
- The generation and properties of transfectants expressing the tk gene of HSV - 1 have been described in detail.
- All transfectants lacked detectable telomerase activity, showed progressive telomere shortening and ultimately arrested and died.
- Stable transfectants with this hypoxia-responsive vector were established using G418 selection for 10 days.
- The antibiotic-resistant colonies were isolated, and a second selection was performed to identify and isolate the transfectants with the highest luciferase expression.

transfection

noun

Microbiology

Now I have a little time before I have to go to a talk, and I'm trying to decide if I should do another set of transfections and start another gel.
Example sentencesExamples
- Cells with double transfections show varying ratios of the species included in the cotransfection but never the species missing from the cotransfection.
- Future studies should reveal other types of well-defined structure-function correlations for transfection in vivo in the presence of serum.
- Then, 4-6 hr after transfection, the cells were washed, fed with new medium, and allowed to grow for 36 hr.
- So, I celebrated by teaching her how to do a transfection, and then we went to lunch and talked about the goods and bads of science, very openly.
- Transient transfection was carried out using lipofectamine according to the protocol suggested by the manufacturer.

Origin

1960s: from trans- 'across' + infect, or a blend of transfer and infect.

Definition of transfect in US English:

transfect

verbtransˈfekt

[with object]Microbiology

1Infect (a cell) with free nucleic acid.
〔微生物〕使（细胞）感染病毒核酸，使转染
cells were transfected by electroporation
Example sentencesExamples
- Cells were grown to a density of 5 x 10 cells/ml and transfected by electroporation.
- MC57 cells were also transfected by electroporation and selected in 1.2 mg/ml G418.
- Stably transfected cells were selected with 500 g/ml G418 (Sigma).
- Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d.
- Cells were transfected with eYFP-C10HRas 3-4 days before the experiment.
1. 1.1 Introduce (genetic material) by transfection.
  用转染法导入（遗传物质）
  Example sentencesExamples
  An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid.
  I have purified, cloned, sub-cloned, transfected, blotted and detected DNA.
  HEK293 cells were cultured in 100 × 20 mm regular culture dishes and transfected with 5 g DNA.
  PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter.
  It was known that there were certain ‘poison sequences’ which might exist upstream of the encoding area of the gene, which would be desirable to remove before transfecting the gene into COS cells.

Origin

1960s: from trans- ‘across’ + infect, or a blend of transfer and infect.

单词	transfect
释义	Definition of transfect in English: transfect verbtranzˈfɛkt-ns-trɑːnz-transˈfekt [with object]Microbiology 1Infect (a cell) with free nucleic acid. 〔微生物〕使（细胞）感染病毒核酸，使转染 cells were transfected by electroporation Example sentencesExamples Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d. Stably transfected cells were selected with 500 g/ml G418 (Sigma). Cells were transfected with eYFP-C10HRas 3-4 days before the experiment. Cells were grown to a density of 5 x 10 cells/ml and transfected by electroporation. MC57 cells were also transfected by electroporation and selected in 1.2 mg/ml G418. 1.1 Introduce (genetic material) by infecting a cell with free nucleic acid. Example sentencesExamples It was known that there were certain ‘poison sequences’ which might exist upstream of the encoding area of the gene, which would be desirable to remove before transfecting the gene into COS cells. PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter. HEK293 cells were cultured in 100 × 20 mm regular culture dishes and transfected with 5 g DNA. I have purified, cloned, sub-cloned, transfected, blotted and detected DNA. An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid. Derivatives transfectant noun Microbiology At 1 ng/ml MMC, no statistically significant difference was observed between wild-type, mutant and vector transfectants. Example sentencesExamples The generation and properties of transfectants expressing the tk gene of HSV - 1 have been described in detail. All transfectants lacked detectable telomerase activity, showed progressive telomere shortening and ultimately arrested and died. Stable transfectants with this hypoxia-responsive vector were established using G418 selection for 10 days. The antibiotic-resistant colonies were isolated, and a second selection was performed to identify and isolate the transfectants with the highest luciferase expression. transfection noun Microbiology Now I have a little time before I have to go to a talk, and I'm trying to decide if I should do another set of transfections and start another gel. Example sentencesExamples Cells with double transfections show varying ratios of the species included in the cotransfection but never the species missing from the cotransfection. Future studies should reveal other types of well-defined structure-function correlations for transfection in vivo in the presence of serum. Then, 4-6 hr after transfection, the cells were washed, fed with new medium, and allowed to grow for 36 hr. So, I celebrated by teaching her how to do a transfection, and then we went to lunch and talked about the goods and bads of science, very openly. Transient transfection was carried out using lipofectamine according to the protocol suggested by the manufacturer. Origin 1960s: from trans- 'across' + infect, or a blend of transfer and infect. Definition of transfect in US English: transfect verbtransˈfekt [with object]Microbiology 1Infect (a cell) with free nucleic acid. 〔微生物〕使（细胞）感染病毒核酸，使转染 cells were transfected by electroporation Example sentencesExamples Cells were grown to a density of 5 x 10 cells/ml and transfected by electroporation. MC57 cells were also transfected by electroporation and selected in 1.2 mg/ml G418. Stably transfected cells were selected with 500 g/ml G418 (Sigma). Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d. Cells were transfected with eYFP-C10HRas 3-4 days before the experiment. 1.1 Introduce (genetic material) by transfection. 用转染法导入（遗传物质） Example sentencesExamples An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid. I have purified, cloned, sub-cloned, transfected, blotted and detected DNA. HEK293 cells were cultured in 100 × 20 mm regular culture dishes and transfected with 5 g DNA. PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter. It was known that there were certain ‘poison sequences’ which might exist upstream of the encoding area of the gene, which would be desirable to remove before transfecting the gene into COS cells. Origin 1960s: from trans- ‘across’ + infect, or a blend of transfer and infect.
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